Rab25 Monoclonal Antibody

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ProSci
Product Code: PSI-32-208
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-208-0.1mg0.1mg£657.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 3F12F3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Rab25 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis using Rab25 monoclonal antibody against truncated Rab25 recombinant protein human overy carcinoma stomach carcinoma breast carcinoma tissue lysate.
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Western blot analysis using Rab25 monoclonal antibody against truncated Rab25 recombinant protein human overy carcinoma stomach carcinoma breast carcinoma tissue lysate.

Documents

Further Information

Additional Names:
CATX8, CATX-8CATX-8, RAB11C
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa.
Background References:
  • JR Goldenring, KR Shen, HD Vaughan. et al. J. Biol. Chem,1993,268(25):18419-18422
  • Xiaoye W, Ravindra K, Jennifer N. et al. J. Biol. Chem,2000,275(37):29138-29146
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified recombinant Rab25 expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
57111
NCBI Official Name:
RAB25, member RAS oncogene family
NCBI Official Symbol:
RAB25
NCBI Organism:
Homo sapiens
Protein Accession #:
P57735
Protein GI Number:
46577696
Purification:
Antibody is purified by protein A affinity chromatography and resuspended in PBS with 0.2%BSA, 0.09% sodium azide.
Research Area:
Signal Transduction
Swissprot #:
P57735
User NOte:
Optimal dilutions for each application to be determined by the researcher.