ELK1 Monoclonal Antibody

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ProSci
Product Code: PSI-32-148
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-148-0.1mL0.1mL£657.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 3H6D12
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
ELK1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis using ELK1 monoclonal antibody against truncated ELK1 recombinant protein and K562 cell lysate.
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Western blot analysis using ELK1 monoclonal antibody against truncated ELK1 recombinant protein and K562 cell lysate.

Documents

Further Information

Additional Names:
ETS domain-containing protein Elk-1
Application Note:
Western Blot:1:500 - 1:1,000
IHC(P):1:500 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
The transcription factor ELK1 is a family of member of ETS oncogene family and of the ternary complex factor (TCF) subfamily,which is located on chromosome Xp11.2 and stimulates transcription. binds to purine-rich DNA sequences. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus,It appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus.
Background References:
  • Rao,V.N., et al. 1989.Science.244 (4900):66-70.
  • Hsieh,Y.H., et al. 2006.Biochem. Biophys. Res. Commun. 339 (1): 217-225.
  • Gille,H., Strahl,T. and Shaw,P.E.1995. Curr. Biol. 5 (10): 1191-1200.
  • Gille,H., et al. 1995.EMBO J. 14 (5): 951-962.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant ELK1 expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
2002
NCBI Official Name:
ELK1, member of ETS oncogene family
NCBI Official Symbol:
ELK1
NCBI Organism:
Homo sapiens
Protein Accession #:
P19419
Protein GI Number:
12643407
Research Area:
Signal Transduction
Swissprot #:
P19419
User NOte:
Optimal dilutions for each application to be determined by the researcher.