GSK3 alpha Monoclonal Antibody

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ProSci
Product Code: PSI-32-164
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-164-0.1mL0.1mL£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Clonality: Monoclonal
Antibody Clone: 6G2F1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
GSK3 alpha monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis using anti - human GSK3 alpha nonoclonal antibody against truncated GSK3 alpha recombinant protein and A549 lysate.
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Western blot analysis using anti - human GSK3 alpha nonoclonal antibody against truncated GSK3 alpha recombinant protein and A549 lysate.

Documents

Further Information

Additional Names:
GSK-3 alpha,
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
GSK3 alpha (Glycogen synthase kinase 3 alpha), with 483-amino acid protein (about 53 kDa), belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily,GSK3 subsubfamily, which also includes GSK3 beta.GSK3 is a multifunctional serine/threonine kinase that is usually inactivated by serine phosphorylation in response to extracellular cues. GSK3 is a key regulator of numerous signalling pathways, including cellular responses to Wnt, receptor tyrosine kinases and G-protein-coupled receptors and is involved in a wide range of cellular processes, ranging from glycogen metabolism to cell cycle regulation and proliferation.GSK3 alpha is implicated in the control of several regulatory proteins including glycogen synthase and transcription factors.
Background References:
  • Bradley W. Doble and James R. Woodgett. J. Cell Sci., Apr 2003; 116: 1175.
  • C. Laura Sayas , Aafke Ariaens, Bas Ponsioen. Mol. Biol. Cell, Apr 2006; 17: 1834 ? 1844.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant GSK3 alpha expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
2931
NCBI Official Name:
glycogen synthase kinase 3 alpha
NCBI Official Symbol:
GSK3A
NCBI Organism:
Homo sapiens
Protein Accession #:
P49840
Protein GI Number:
12644292
Research Area:
Signal Transduction
Swissprot #:
P49840
User NOte:
Optimal dilutions for each application to be determined by the researcher.