P16 Monoclonal Antibody

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ProSci
Product Code: PSI-32-172
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-172-0.1mL0.1mL£560.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Clonality: Monoclonal
Antibody Clone: 5A8A4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Human P16 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis using P16 monoclonal antibody against truncated P16 recombinant protein.
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Western blot analysis using P16 monoclonal antibody against truncated P16 recombinant protein.

Documents

Further Information

Additional Names:
ARF, MLM, P14, P16, P19, CMM2, INK4, MTS1, TP16, CDK4I, CDKN2, INK4A, MTS-1, P14ARF, P19ARF, P16INK4, P16INK4A, P16-INK4A
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
p16 (cyclin-dependent kinase inhibitor 2A, INK4a) is a tumor suppressor protein. It is a specific inhibitor of Cdk 4 / Cdk 6, and a tumor suppressor involved in the pathogenesis of a variety of malignancies. Recent analyses of the p16 INK4a gene revealed homozygous deletions, nonsense, missense, or frameshift mutations in several human cancers. Although the frequency of p16 INK4a abnormalities is higher in tumor derived cell lines than in unselected primary tumors, significant subsets of clinical cases with aberrant p16 INK4a gene have been reported among melanomas, gliomas, esophageal, pancreatic, lung, and urinary bladder carcinomas, and some types of leukemia.
Background References:
  • Bai, F. et al. Mol. Cell. Biol.2003 23, 1269-1277.
  • Lowe, S.W. and Sherr, C.J. Curr. Opin. Genet.2003 Dev.13, 77-83.
  • Sherr, C.J. Nat. Rev. Mol. Cell Biol.2001 2, 731-737.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant P16 expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
1029
NCBI Official Name:
cyclin-dependent kinase inhibitor 2A
NCBI Official Symbol:
CDKN2A
NCBI Organism:
Homo sapiens
Protein Accession #:
NP_000068.1
Protein GI Number:
4502749
Swissprot #:
P42771
User NOte:
Optimal dilutions for each application to be determined by the researcher.