Proenkephalin Antibody

ProSci
Product Code: PSI-46-227
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-227-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-227 (0.3ug/ml) staining of Human Adrenal Gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-227 (0.3ug/ml) staining of Human Adrenal Gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
proenkephalin, PENK
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37kDa band observed in Human Adrenal Gland lysates (calculated MW of 30.8kDa according to NP_006202.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Normant and Loh, Endocrinology. 1998 Apr;139(4):2137-45; PMID: 9529003). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Frozen section of Human Hypothalamus shows staining of dense enkephalinergic axon plexus and scattered neuronal cell bodies (higher magnification inset) in the human infundibular nucleus. Recommended concentration: 0.3-1ug/ml.
Background References:
  • Schiltz CA, Bremer QZ, Landry CF, Kelley AE. Food-associated cues alter forebrain functional connectivity as assessed with immediate early gene and proenkephalin expression. BMC Biol. 2007 Apr 26;5:16.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-RSHHQDGSDNEE
NCBI Gene ID #:
5179
NCBI Official Name:
proenkephalin
NCBI Official Symbol:
PENK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006202.1
Protein GI Number:
5453876
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P01210