PTB Antibody

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ProSci
Product Code: PSI-46-243
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-243-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-243 (0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-243 (0.03ug/ml) staining of lysates of cell line NIH3T3 (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-243 (0.1-0.3ug/ml) staining of Mouse Ovary (A) and Rat Ovary (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-243 (0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-243 (0.03ug/ml) staining of lysates of cell line NIH3T3 (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-243 (0.1-0.3ug/ml) staining of Mouse Ovary (A) and Rat Ovary (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
PTBP1, PTB, polypyrimidine tract binding protein 1, PTB2, PTB3, PTB4, pPTB, HNRPI, PTB-1, HNRNPI, RNA binding protein, heterogeneous nuclear ribonucleoprotein polypeptide I, polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I), HNRNP-I, MGC10830, MGC8461, RNA-binding protein, heterogeneous nuclear ribonucleoprotein I
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Human, Mouse and Rat Ovary lysates and 60kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat, HEK293 and 3T3N (calculated MW of 57.2kDa according to NP_114368.1). Recommended concentration: 0.01-0.3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Liver and Ovary for this particular batch.Immunohistochemistry:In paraffin embedded Human Kidney shows nuclear membrane staining in all the convoluted tubules. Recommended concentration: 2-4ug/ml.
Background References:
  • Charlet-B N, Logan P, Singh G, Cooper TA. Dynamic antagonism between ETR-3 and PTB regulates cell type-specific alternative splicing. Mol Cell. 2002 Mar;9(3):649-58.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: DGIVPDIAVGTKR-C
NCBI Gene ID #:
19205 (mouse); 29497 (rat);
NCBI Official Name:
polypyrimidine tract binding protein 1
NCBI Official Symbol:
PTBP1
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
NP_002810.1, NP_114367.1, NP_114368.1, NP_787041.1
Protein GI Number:
4506243
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise all 4 reported isoforms (represented by NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1).
Swissprot #:
P17225