RASIP1 Antibody

ProSci
Product Code: PSI-46-272
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-272-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-272 (3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

46-272 (3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
RASIP1, Ras-interacting protein 1, RAIN, FLJ20401, Ras interacting protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Experiments gave bands at approx 95kDa, 75kDa and 45kDa in A549 Lung Carcinoma lysate after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the predicted size of 110kDa according to NP_060275. The detected bands were successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 3.75ug/ml.
Background References:
  • Natalia Y. Mitin, Melissa B. Ramocki, Alfred J. Zullo, Channing J. Der, Stephen F. Konieczny, and Elizabeth J. Taparowsky Identification and Characterization of Rain, a Novel Ras-interacting Protein with a Unique Subcellular Localization J. Biol. Chem. 2004;279 22353-22361
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: CANYRHGPPVATSP
NCBI Gene ID #:
54922
NCBI Official Name:
Ras interacting protein 1
NCBI Official Symbol:
RASIP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_060275.2
Protein GI Number:
38570105
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
Q5U651