SAFB2 Antibody

ProSci
Product Code: PSI-42-095
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-095-0.1mg0.1mg£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Further Information

Additional Names:
SAFB2, scaffold attachment factor B2, KIAA0138
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 75kDa and 60kDa in lysates of HeLa cell lines after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 107kDa according to NP_055464.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Oesterreich S. Scaffold attachment factors SAFB1 and SAFB2: Innocent bystanders or critical players in breast tumorigenesis J Cell Biochem. 2003 Nov 1;90(4):653-61.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-GEHNQRLEEHQARA
NCBI Gene ID #:
9667
NCBI Official Name:
scaffold attachment factor B2
NCBI Official Symbol:
SAFB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055464.1
Protein GI Number:
7661936
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
This antibody is not expected to cross-react with SAFB1.
Swissprot #:
Q14151