Semaphorin 5A Antibody
| Code | Size | Price |
|---|
| PSI-46-349-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
SEMA5A, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A, HGNC:10736, SEMAF, semF, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 90kDa in Mouse Brain lysates and approx 25kDa band in Human Brain (Cerebellum), Colon, Heart and Kidney lysates after 0.03ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 121kDa according to NP_003957.1. The 90kDa and 25kDa band were successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Kidney shows membanous and vesicular staining in PCT cells. Recommended concentration: 4-6ug/ml.
Background References:
- Maraganore DM, de Andrade M, Lesnick TG, Strain KJ, Farrer MJ, Rocca WA, Pant PV, Frazer KA, Cox DR, Ballinger DG. High-resolution whole-genome association study of Parkinson disease Am J Hum Genet. 2005 Nov;77(5):685-93. Epub 2005 Sep 9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: DEATKKACYSKGKS
NCBI Gene ID #:
9037
NCBI Official Name:
sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
NCBI Official Symbol:
SEMA5A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003957.1
Protein GI Number:
4506881
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
The immunizing peptide corresponds to a part of the extracellular domain.
Swissprot #:
Q13591