Septin 3 Antibody

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ProSci
Product Code: PSI-46-353
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-353-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-353 staining (1ug/ml) of Human Brain lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
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46-353 (3.75ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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46-353 staining (1ug/ml) of Human Brain lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
46-353 (3.75ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
SEPT3, septin 3, SEP3, CTA-250D10.3, MGC133218, bK250D10.3, OTTHUMP00000028742, neuronal-specific septin 3, G-Septin
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa band seen in Human Brain lysate. Recommended for use at 0.5-2ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and (Cerebellum) and Testis. Recommended concentration: 3.75ug/ml.
Background References:
  • Xue J, Tsang CW, Gai WP, Malladi CS, Trimble WS, Rostas JA, Robinson PJ. Septin 3 (G-septin) is a developmentally regulated phosphoprotein enriched in presynaptic nerve terminals. J Neurochem. 2004 Nov;91(3):579-90.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: SELVPEPRPKPA-C
NCBI Gene ID #:
55964
NCBI Official Name:
septin 3
NCBI Official Symbol:
Sep-03
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_663786.1, NP_061979.3
Protein GI Number:
22035572
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize both reported isoforms according to NP_663786.1 and NP_061979.3.
Swissprot #:
Q9UH03