TCFL5 Antibody

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ProSci
Product Code: PSI-46-475
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-475-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-475 (0.3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
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46-475 (0.3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
TCFL5, CHA, transcription factor-like 5 (basic helix-loop-helix), E2BP-1, Figlb, MGC46135 , HPV-16 E2 binding protein 1, transcription factor-like 5 protein, Cha transcription factor
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 55kDa band observed in Human Testis lysates (calculated MW of 52.7kDa according to NP_006593.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was greatly reduced by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Siep M, Sleddens-Linkels E, Mulders S, van Eenennaam H, Wassenaar E, Van Cappellen WA, Hoogerbrugge J, Grootegoed JA, Baarends WM. Basic helix-loop-helix transcription factor Tcfl5 interacts with the Calmegin gene promoter in mouse spermatogenesis. Nucleic Acids Res. 2004 Dec 7;32(21):6425-36. Print 2004.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-KYIQERHGDSLKKE
NCBI Gene ID #:
10732
NCBI Official Name:
transcription factor-like 5 (basic helix-loop-helix)
NCBI Official Symbol:
TCFL5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006593.2
Protein GI Number:
38505159
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9UL49