TIA1 Antibody
| Code | Size | Price |
|---|
| PSI-46-492-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
TIA1, TIA1 cytotoxic granule-associated RNA binding protein , TIA1 cytotoxic granule-associated RNA-binding protein, TIA1 protein, cytotoxic granule-associated RNA-binding protein, p40-TIA-1 (containing p15-TIA-1)
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in lysates of cell line Jurkat (calculated MW of 41.8kDa according to NP_071320.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
An additional band at 80kDa and a very strong 26kDa is also observed. These bands were successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Thyroid. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of HeLa cells. Recommended concentration: 5ug/ml.
Background References:
- Yang F, Peng Y, Murray EL, Otsuka Y, Kedersha N, Schoenberg DR. Polysome-bound endonuclease PMR1 is targeted to stress granules via stress-specific binding to TIA-1. Mol Cell Biol. 2006 Dec;26(23):8803-13. Epub 2006 Sep 18.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-PKSTYESNTKQ
NCBI Gene ID #:
7072
NCBI Official Name:
TIA1 cytotoxic granule-associated RNA binding protein
NCBI Official Symbol:
TIA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_071320.1, NP_071505.1
Protein GI Number:
11863161
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_071320.1 and NP_071505.1).
Swissprot #:
P31483