TIM-3 Antibody

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ProSci
Product Code: PSI-46-495
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-495-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-495 (2ug/ml) staining of Jurkat (A), MOLT4 (B) and negative control A431 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-495 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo
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46-495 (2ug/ml) staining of Jurkat (A), MOLT4 (B) and negative control A431 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-495 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo

Documents

Further Information

Additional Names:
HAVCR2, TIM3, hepatitis A virus cellular receptor 2, FLJ14428, KIM-3, TIMD3, Tim-3, T cell immunoglobulin mucin 3, T cell immunoglobulin mucin-3, kidney injury molecule-3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in lysates of cell lines Jurkat and MOLT-4 and in Human Tonsil lysates (calculated MW of 33.4kDa according to NP_116171.3). Recommended concentration:1-3ug/ml. Primary incubation 1 hour at room temperature. Negative Control: A431 (squamous carcinoma) cell lysate.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/membranes and nuclei of HepG2 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Background References:
  • Meyers JH, Sabatos CA, Chakravarti S, Kuchroo VK. The TIM gene family regulates autoimmune and allergic diseases. Trends Mol Med. 2005 Aug;11(8):362-9. Review.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-KWYSHSKEKIQN
NCBI Gene ID #:
84868
NCBI Official Name:
hepatitis A virus cellular receptor 2
NCBI Official Symbol:
HAVCR2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_116171.3
Protein GI Number:
49574534
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q8TDQ0