TPD52 Antibody

ProSci
Product Code: PSI-46-506
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-506-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-506 (1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-506 (1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
TPD52, tumor protein D52, D52, N8L, PC-1, PrLZ, hD52, PC1/MRPS28 fusion protein, prostate and colon associated protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 26-28kDa band observed in Human Lymph Node and Human Kidney lysates (calculated MW of 24.3kDa according to NP_001020423.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tiacci et al , Blood. 2005 Apr 1;105(7):2812-20.; PMID: 15576473). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.
Background References:
  • Byrne JA, Balleine RL, Schoenberg Fejzo M, Mercieca J, Chiew YE, Livnat Y, St Heaps L, Peters GB, Byth K, Karlan BY, Slamon DJ, Harnett P, Defazio A. Tumor protein D52 (TPD52) is overexpressed and a gene amplification target in ovarian cancer. Int J Cancer. 2005 Dec 20;117(6):1049-54.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-TEPLPEKTQES
NCBI Gene ID #:
7163
NCBI Official Name:
tumor protein D52
NCBI Official Symbol:
TPD52
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001020423.1 , NP_001020424.1, NP_005070.1
Protein GI Number:
70608172
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise all three reported isoforms (NP_001020423.1, NP_001020424.1and NP_005070.1).
Swissprot #:
P55327