TRIM2 Antibody

ProSci
Product Code: PSI-46-519
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-519-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-519 (0.5ug/ml) staining of Rat Brain (A) + Peptide (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-519 (0.5ug/ml) staining of Rat Brain (A) + Peptide (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
TRIM2, tripartite motif-containing 2, KIAA0517, tripartite motif protein 2, tripartite motif protein TRIM2, RNF86, tripartite motif protein TRIM2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75-85kDa band observed in Rat Brain lysates (calculated MW of 83.3 according to Rat NP_001102022.1). An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.5-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Brain (Cortex) shows cytoplasm stainning in neuronal cell bodies. Recommended concentration: 6-12ug/ml.Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
Background References:
  • Reymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L, Riganelli D, Zanaria E, Messali S, Cainarca S, Guffanti A, Minucci S, Pelicci PG, Ballabio A. The tripartite motif family identifies cell compartments. EMBO J. 2001 May 1;20(9):2140-51.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: DSGNHCFKVYRYLQ
NCBI Gene ID #:
23321
NCBI Official Name:
tripartite motif containing 2
NCBI Official Symbol:
TRIM2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_056086.2, NP_001123539.1, NP_001337983.1, NP_001337984.1, NP_001337985.1l, NP_001337986.1
Protein GI Number:
194248079
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
Cross-reactivity with TRIM3 has been demonstrated in Mouse.
Swissprot #:
Q9C040