VPS11 Antibody
| Code | Size | Price |
|---|
| PSI-46-575-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
VPS11, vacuolar protein sorting 11 (yeast) , HGNC:14583, END1, PEP5, RNF108, hVPS11 , vacuolar protein sorting 11 (yeast homolog), vacuolar protein sorting protein 11, vacuolar protein sorting 11 homolog (S. cerevisiae)
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 75kDa band in human duodenum, colon and placenta lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 108kDa according to NP_068375.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Kidney shows brushborder staning in PCT cells and Human Pancreas shows cytoplasm staining of beta cells in islets of Langerhans. Recommended concentration, 3-6ug/ml.
Background References:
- Kim BY, Kramer H, Yamamoto A, Kominami E, Kohsaka S, Akazawa C. Molecular characterization of mammalian homologues of class C Vps proteins that interact with syntaxin-7. J Biol Chem. 2001 Aug 3;276(31):29393-402. Epub 2001 May 29.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-HALQEKDTQTKLE
NCBI Gene ID #:
55823
NCBI Official Name:
vacuolar protein sorting 11 homolog (S. cerevisiae)
NCBI Official Symbol:
VPS11
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_068375.3
Protein GI Number:
17978477
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
Q9H270