VTCN1 Antibody

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ProSci
Product Code: PSI-46-589
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-589-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-589 (1ug/ml) staining of HEK293 iso 1 + iso 2 cell lysate (A) + peptide (B). Human Adrenal Gland iso 1 (C), Human Adrenal Gland iso 2 (D) and (0.1ug/ml) Human pancreas iso 1 (E) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-589 (1ug/ml) staining of HEK293 iso 1 + iso 2 cell lysate (A) + peptide (B). Human Adrenal Gland iso 1 (C), Human Adrenal Gland iso 2 (D) and (0.1ug/ml) Human pancreas iso 1 (E) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
B7-H4, B7H4, B7S1, B7X, B7h.5, FLJ22418, PRO1291, RP11-229A19.4, VCTN1, T cell costimulatory molecule B7x, immune costimulatory protein, V-set domain containing T cell activation inhibitor 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 26+21kDa bands observed in HEK293 cell lysates, which were both successfully blocked by incubation with the immunizing peptide. An approx 26kDa and 21kDa band observed in Human Adrenal Gland lysates, and approx 27kDa in Human Pancreas lysates (calculated MW of 30.9kDa according to NP_078902.2 isoform 1 and 20.7kDa according to NP_001240778.1 isoform 2 ). Recommended concentration: 0.1-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes/cell junctions of MCF7 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Background References:
  • Krambeck AE, Thompson RH, Dong H, Lohse CM, Park ES, Kuntz SM, Leibovich BC, Blute ML, Cheville JC, Kwon ED. B7-H4 expression in renal cell carcinoma and tumor vasculature: associations with cancer progression and survival. Proc Natl Acad Sci USA. 2006 Jul 5;103(27):10391-6. Epub 2006 Jun 23.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-SKGKGNANLEYK
NCBI Gene ID #:
79679
NCBI Official Name:
V-set domain containing T cell activation inhibitor 1
NCBI Official Symbol:
VTCN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_078902.2, NP_001240778.1
Protein GI Number:
99028881
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q7Z7D3