WISP1 Antibody

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ProSci
Product Code: PSI-46-597
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-597-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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HEK293 overexpressing WIS with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with 46-597 in the right panel (mock transfection in first lane in each panel).
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HEK293 overexpressing WIS with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with 46-597 in the right panel (mock transfection in first lane in each panel).

Documents

Further Information

Additional Names:
WISP1, WNT1 inducible signaling pathway protein 1, CCN4, WISP1c, WISP1i, WISP1tc, WNT1 induced secreted protein 1, Wnt-1 inducible signaling pathway protein 1, wnt-1 signaling pathway protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:In transfected HEK293 transiently expressing WISP1 a band of approx. 35kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • Major MB, Camp ND, Berndt JD, Yi X, Goldenberg SJ, Hubbert C, Biechele TL, Gingras AC, Zheng N, Maccoss MJ, Angers S, Moon RT. Wilms tumor suppressor WTX negatively regulates WNT/beta-catenin signaling. Science. 2007 May 18;316(5827):1043-6.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat,
Immunogen:
The immunogen for this antibody is: C-ESYPDFSEIAN
NCBI Gene ID #:
8840
NCBI Official Name:
WNT1 inducible signaling pathway protein 1
NCBI Official Symbol:
WISP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003873.1, NP_543028.1
Protein GI Number:
4507921
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_003873.1; NP_543028.1).
Swissprot #:
O95388