ZASP Antibody

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ProSci
Product Code: PSI-46-608
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-608-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-608 (0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-608 (0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
LDB3, ZASP, LIM domain binding 3 , HGNC:15710, KIAA01613, KIAA0613, ORACLE, PDLIM6, PDZ and LIM domain 6, Z-band alternatively spliced PDZ-motif, CYPHER, FLJ35865, ldb3z1, ldb3z4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Western Blot experiments gave bands at approx 90kDa and 35kDa in Human Skeletal Muscle lysate after 1ug/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Huang et al J Biol Chem. 2003 Feb 28;278(9):7360-5; PMID: 12499364). This protein has a calculated MW of 77.1kDa according to NP_009009.1. Recommended concentration: 0.1-1ug/ml.
Background References:
  • Selcen D, Engel AG. Mutations in ZASP define a novel form of muscular dystrophy in humans. Ann Neurol. 2005 Feb;57(2):269-76.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: C-KSQNKPEDEADE
NCBI Gene ID #:
11155
NCBI Official Name:
LIM domain binding 3
NCBI Official Symbol:
LDB3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_009009.1, NP_001073583.1, NP_001073584.1, NP_001073585.1
Protein GI Number:
45592959
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
O75112