GATA3 Antibody

ProSci
Product Code: PSI-25-031
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-25-031-100ul100ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store GATA3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in IHC on Human kidney lysate at 5.0 ug/ml.
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Antibody used in WB on Hum. Fetal Heart at 1 ug/ml.
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Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
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Antibody used in WB on MCF7 at 1 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
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Antibody used in WB on Human MCF7 cells at 1 ug/ml.

Antibody used in IHC on Human kidney lysate at 5.0 ug/ml.
Antibody used in WB on Hum. Fetal Heart at 1 ug/ml.
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
Antibody used in WB on MCF7 at 1 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Antibody used in WB on Human MCF7 cells at 1 ug/ml.

Further Information

Additional Names:
GATA3, HDR, MGC2346, MGC5199, MGC5445, HDRS
Application Note:
GATA3 antibody can be used for detection of GATA3 by ELISA at 1:1562500. GATA3 antibody can be used for detection of GATA3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
GATA3 is crucially involved in IL-5 gene transcription in human peripheral CD4-positive t cells. This gene is involved in growth control and the maintenance of the differentiated state in epithelial cells and may contribute to tumorigenesis in breast cancer.
Background References:
  • Arshad, S.H., (2008) Br. J. Dermatol. 158 (6), 1315-1322.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GATA3.
NCBI Gene ID #:
2625
NCBI Official Name:
GATA binding protein 3
NCBI Official Symbol:
GATA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
Protein Accession #:
NP_002042
Protein GI Number:
4503929
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Transcription ,Cancer
Swissprot #:
P23771
User NOte:
Optimal dilutions for each application to be determined by the researcher.