HMGB1 Antibody

ProSci
Product Code: PSI-28-325
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-28-325-100ul100ul£609.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HMGB1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.

Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.

Further Information

Additional Names:
HMGB1, HMG1, HMG3, SBP-1
Application Note:
HMGB1 antibody can be used for detection of HMGB1 by ELISA at 1:62500. HMGB1 antibody can be used for detection of HMGB1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
Extracellular HMGB1 is an activator of human tumor cell migration operating in concert with EGF. HMGB1 encodes a protein that is potentially involved in the regulation of lipogenic and cholesterogenic gene transcription.
Background References:
  • Messmer, D., et al., (2004) J. Immunol. 173 (1), 307-313.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HMGB1.
NCBI Gene ID #:
3146
NCBI Official Name:
high mobility group box 1
NCBI Official Symbol:
HMGB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
25 kDa, 24 kDa
Protein Accession #:
NP_002119
Protein GI Number:
4504425
Research Area:
Transcription ,Cancer
Swissprot #:
Q5T7C3
User NOte:
Optimal dilutions for each application to be determined by the researcher.