MBD2 Antibody
| Code | Size | Price |
|---|
| PSI-29-201-100ul | 100ul | £609.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store MBD2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
MBD2, DMTase, NY-CO-41
Application Note:
MBD2 antibody can be used for detection of MBD2 by ELISA at 1:1562500. MBD2 antibody can be used for detection of MBD2 by western blot at 0.625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
MBD2 belongs to a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD2 can also repress transcription from methylated gene promoters. MBD2 may function as a mediator of the biological consequences of the methylation signal.It is also reported that the MBD2 functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.
Background References:
- Le (2006) Mol. Cell. Biol. 26 (3), 843-851.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MBD2.
NCBI Gene ID #:
8932
NCBI Official Name:
methyl-CpG binding domain protein 2
NCBI Official Symbol:
MBD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
32 kDa
Protein Accession #:
NP_056647
Protein GI Number:
7710145
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Transcription ,Cancer
Swissprot #:
Q9UBB5-3
User NOte:
Optimal dilutions for each application to be determined by the researcher.