NKX2-8 Antibody

ProSci
Product Code: PSI-27-420
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-27-420-100ul100ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NKX2-8 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

1 / 5
Antibody used in WB on Human Heart lysate at 0.2-1 ug/ml.
2 / 5
3 / 5
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.
4 / 5
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
5 / 5
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.

Antibody used in WB on Human Heart lysate at 0.2-1 ug/ml.
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.

Further Information

Additional Names:
NKX2-8, NKX2.8, NKX2H, Nkx2-9
Application Note:
NKX2-8 antibody can be used for detection of NKX2-8 by ELISA at 1:12500. NKX2-8 antibody can be used for detection of NKX2-8 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
A murine NKX2-8 was isolated from the Hepal-6 cell line and showed oligonucleotide binding competitive with fetoprotein transcription factor. Nkx2.8 bound to the active AFP promoter, and antisense inhibition of Nkx2.8 mRNA translation selectively reduced expression of both the endogenous human AFP gene and transfected reporters containing the rat AFP promoter.
Background References:
  • Hamamoto, R., (2004) Nat. Cell Biol. 6 (8), 731-740.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NKX2-8.
NCBI Gene ID #:
26257
NCBI Official Name:
NK2 homeobox 8
NCBI Official Symbol:
NKX2-8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
26 kDa
Protein Accession #:
NP_055175
Protein GI Number:
31377777
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Transcription ,Cancer
Swissprot #:
O15522
User NOte:
Optimal dilutions for each application to be determined by the researcher.