PAX7 Antibody

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ProSci
Product Code: PSI-27-583
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-27-583-100ul100ul£662.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PAX7 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in IHC on Human Skin at 5.0 ug/ml.
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3 / 5
Antibody used in WB on Mouse skeletal muscle at: 1:1000 (Lanes: 1: Mouse skeletal muscle lysate, 2: Mouse skeletal muscle lysate, 3: Mouse skeletal muscle lysate).
4 / 5
Antibody used in WB on Human Jurkat 0.05 ug/ml.
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Antibody used in WB on Jurkat 0.0625 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
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Antibody used in IHC on Human Skin at 5.0 ug/ml.
Antibody used in WB on Mouse skeletal muscle at: 1:1000 (Lanes: 1: Mouse skeletal muscle lysate, 2: Mouse skeletal muscle lysate, 3: Mouse skeletal muscle lysate).
Antibody used in WB on Human Jurkat 0.05 ug/ml.
Antibody used in WB on Jurkat 0.0625 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).

Documents

Further Information

Additional Names:
PAX7, HUP1, RMS2, PAX7B
Application Note:
PAX7 antibody can be used for detection of PAX7 by ELISA at 1:312500. PAX7 antibody can be used for detection of PAX7 by western blot at 0.05 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
PAX7 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box gene 7 is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Alternative splicing in this gene has produced two known products but the biological significance of the variants is unknown.
Background References:
  • Du, S., et al., (2005) Int. J. Cancer 115 (1), 85-92.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PAX7.
NCBI Gene ID #:
5081
NCBI Official Name:
paired box 7
NCBI Official Symbol:
PAX7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
57 kDa, 57 kDa
Protein Accession #:
NP_002575
Protein GI Number:
4505619
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Transcription ,Cancer
Swissprot #:
P23759
User NOte:
Optimal dilutions for each application to be determined by the researcher.