POU1F1 Antibody
| Code | Size | Price |
|---|
| PSI-27-337-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Canine (dog)
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store POU1F1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
POU1F1, PIT1, CPHD1, GHF-1, Pit-1, POU1F1a
Application Note:
POU1F1 antibody can be used for detection of POU1F1 by ELISA at 1:12500. POU1F1 antibody can be used for detection of POU1F1 by western blot at 1.0-10.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
PIT1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals and is a member of the POU family of transcription factors that regulate mammalian development. The POU family is so named because the first 3 members identified were PIT1 and OCT1 of mammals, and Unc-86 of C. elegans. PIT1 contains 2 protein domains, termed POU-specific and POU-homeo, which are both necessary for high affinity DNA binding on genes encoding growth hormone and prolactin. PIT1 is also important for regulation of the genes encoding prolactin and thyroid-stimulating hormone beta subunit by thyrotropin-releasing hormone and cyclic AMP.
Background References:
- Dattani, M.T., et al., (2003) GrowthHorm.IGFRes.16(9), 1207-1209.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POU1F1.
NCBI Gene ID #:
5449
NCBI Official Name:
POU class 1 homeobox 1
NCBI Official Symbol:
POU1F1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
33 kDa
Protein Accession #:
NP_000297
Protein GI Number:
4505955
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Transcription ,Cancer
Swissprot #:
P28069
User NOte:
Optimal dilutions for each application to be determined by the researcher.