SPDEF Antibody

ProSci
Product Code: PSI-27-414
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-27-414-100ul100ul£609.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SPDEF antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human MCF7 cells at 2.5 ug/ml.

Antibody used in WB on Human MCF7 cells at 2.5 ug/ml.

Further Information

Additional Names:
SPDEF, PDEF, RP11-375E1__A.3, bA375E1.3
Application Note:
SPDEF antibody can be used for detection of SPDEF by ELISA at 1:12500. SPDEF antibody can be used for detection of SPDEF by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
PDEF is an ETS transcription factor expressed in prostate epithelial cells. It acts as an androgen-independent transactivator of PSA expression.PDEF is an ETS transcription factor expressed in prostate epithelial cells. It acts as an androgen-independent transactivator of PSA (MIM 176820) expression.
Background References:
  • Fowler, M., (2006) J. Cell. Biochem. 97 (1), 1-17.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPDEF.
NCBI Gene ID #:
25803
NCBI Official Name:
SAM pointed domain containing ETS transcription factor
NCBI Official Symbol:
SPDEF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
NP_036523
Protein GI Number:
6912580
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Transcription ,Cancer
Swissprot #:
O95238
User NOte:
Optimal dilutions for each application to be determined by the researcher.