CHRNB4 Antibody
| Code | Size | Price |
|---|
| PSI-46-825-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
CHRNB4 , cholinergic receptor, nicotinicN: beta 4 , Cholinergic receptor, neuronal nicotinic, beta polypeptide-4, cholinergic receptor, nicotinic, beta polypeptide 4, neuronal nicotinic receptor beta 4 subunit
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 40kDa band in human testis lysates after 0.2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4kDa according to NP_000741.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 2.5ug/ml.
Background References:
- Liang Y, Salas R, Marubio L, Bercovich D, De Biasi M, Beaudet AL, Dani JA. Functional polymorphisms in the human beta4 subunit of nicotinic acetylcholine receptors. Neurogenetics. 2005 Feb;6(1):37-44. Epub 2004 Nov 25.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-DYRLTWNSSRYEGVN
NCBI Gene ID #:
1143
NCBI Official Name:
cholinergic receptor, nicotinic, beta 4 (neuronal)
NCBI Official Symbol:
CHRNB4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000741.1
Protein GI Number:
4502837
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
SPECIFICITY:
The immunizing peptide represents part of the first extracellular domain (aa22-236).
Swissprot #:
P30926