IgG Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-32-176-0.1mg | 0.1mg | £404.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 4D2D9G8
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
IgG monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
CD32, FCG2, FCGR2A1, IGFR2, Low affinity immunoglobulin gamma Fc region receptor II-a, CDw32, IgG Fc receptor II-a, FcGR, CD32A, FCGR2
Application Note:
Western Blot:1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
Background References:
- Roitt,I, et al "Immunology", Mosby, London, England, (1996) fourth edition.
- Vlug, A. et al "The structure and function of human IgG subclasses". Eur.Clin.Lab. 8, 26 (1989).
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Chrompure Human IgG, Fc Fragment.
NCBI Gene ID #:
2212
NCBI Official Name:
Fc fragment of IgG, low affinity IIa, receptor (CD32)
NCBI Official Symbol:
FCGR2A
NCBI Organism:
Homo sapiens
Protein Accession #:
P12318
Protein GI Number:
160332371
Purification:
Antibody is purified by protein A affinity chromatography.
Research Area:
Immunology
Swissprot #:
P12318
User NOte:
Optimal dilutions for each application to be determined by the researcher.