Desmin Antibody

Contact us
ProSci
Product Code: PSI-46-777
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-777-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 5
46-777 (1ug/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
2 / 5
3 / 5
4 / 5
46-777 (3.75ug/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
5 / 5
46-777 Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.
❮❯

46-777 (1ug/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-777 (3.75ug/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
46-777 Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.

Documents

Further Information

Additional Names:
DES, desmin, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, intermediate filament protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 55-60kDa band observed in Human, Mouse, Rat and Pig Skeletal Muscle lysates (calculated MW of 53.5kDa according to Human NP_001918.3, Mouse NP_034173.1, Rat NP_071976.1 and 53.6kDa according to Pig NP_001001535.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Heart and Skeletal Muscle. Recommended concentration: 5ug/ml. In paraffin embedded Human Heart, expression of Desmin is detected in myocardial muscle and not in adjacent vascular smooth muscle. Recommended concentration:3.75ug/ml. Data obtained from previous batch.
Background References:
  • Chen F, Chang R, Trivedi M, Capetanaki Y, Cryns VL. Caspase proteolysis of desmin produces a dominant-negative inhibitor of intermediate filaments and promotes apoptosis. J Biol Chem. 2003 Feb 28;278(9):6848-53.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-RDGEVVSEATQQQHE
NCBI Gene ID #:
1674
NCBI Official Name:
desmin
NCBI Official Symbol:
DES
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001918.3
Protein GI Number:
55749932
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Stem Cell
SPECIFICITY:
.
Swissprot #:
P17661