Melanophilin Antibody

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ProSci
Product Code: PSI-45-882
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-882-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • GST-PD
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-882 staining (0.5ug/ml) of total melanocyte PNS (200ug per lane) of wild-type and leaden (Mlph null) mice. The upper band may represent the stacking-separating gel interface. Data kindly provided by A N Hume, Imperial College.
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45-882 staining (0.5ug/ml) of total melanocyte PNS (200ug per lane) of wild-type and leaden (Mlph null) mice. The upper band may represent the stacking-separating gel interface. Data kindly provided by A N Hume, Imperial College.

Documents

Further Information

Additional Names:
2210418F23Rik, melanophilin, Mlph, ln, l1Rk3, Slac-2a, D1Wsu84e, l(1)-3Rk, 5031433I09Rik, leaden, AW228792, OTTMUSP00000025451
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80 kDa band observed in Mouse Melanocyte extracts. Recommended for use at 0.5-2ug/ml. Please note that this product may require overnight incubation of primary and high protein loads. This antibody has been successfully used in WB on Mouse: Sanz? Pet al (PMID: 30898842), Singh RK et al (PMID: 23281710), Figueiredo AC et al (PMID: 18559336) and Hume AN et al (PMID: 16914517).Immunofluorescence: Successfully used at 0.5ug/ml to stain mouse melanocytes. This antibody has been successfully used in IF on Mouse: Figueiredo AC et al., J Biol Chem. 2008 Aug 22;283(34):23209-16. PMID:18559336.
GST-pull down assay: See publication Figueiredo AC et al., J Biol Chem. 2008 Aug 22;283(34):23209-16. (PMID:18559336).
Background References:
  • Matesic LE, Yip R, Reuss AE, Swing DA, O'Sullivan TN, Fletcher CF, Copeland NG, Jenkins NA. Mutations in Mlph, encoding a member of the Rab effector family, cause the melanosome transport defects observed in leaden mice. Proc Natl Acad Sci USA. 2001 Aug 28;98(18):10238-43.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-ARHIFAKPVMAQQP
NCBI Gene ID #:
171531 (mouse); 316620 (rat);
NCBI Official Name:
melanophilin
NCBI Official Symbol:
Mlph
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
NP_443748.2
Protein GI Number:
87080831
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
Q91V27