MID2 Antibody

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ProSci
Product Code: PSI-45-887
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-887-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-887 (0.1ug/ml) staining of Mouse (A) and Rat (B) Heart, and Rat Kidney (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-887 (0.1ug/ml) staining of Mouse (A) and Rat (B) Heart, and Rat Kidney (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
MID2, TRIM1, midline 2, FXY2, midin 2, tripartite motif protein 1, RP6-191P20.2, FLJ37715, FLJ41813, FXY2, RNF60,
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80-85kDa band observed in Mouse and Rat Heart, and Rat Kidney lysates (calculated MW of 77.8kDa according to Mouse NP_035975.1 and 83.1kDa according to Rat NP_001178818.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows strong staining in cytoplasm and brush border of some epithelial cells of proximal convoluted tubules. Recommended concentration, 5-10ug/ml.
Background References:
  • Buchner G, Montini E, Andolfi G, Quaderi N, Cainarca S, Messali S, Bassi MT, Ballabio A, Meroni G, Franco B. MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development. Hum Mol Genet. 1999 Aug;8(8):1397-407.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: QESPYVSGMKTCH
NCBI Gene ID #:
11043
NCBI Official Name:
midline 2
NCBI Official Symbol:
MID2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_036348.2, NP_438112.2
Protein GI Number:
6912504
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both human isoforms of this protein.
Swissprot #:
Q9UJV3