NPT Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-32-199-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 4B4D1
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
NPT monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Application Note:
Western Blot:Dilution 1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.
Background References:
- Landis WG et.al Human and ecological Risk Assessment 6(5): 875-899.
- Stewart CN et.al BioTechniques 29(4): 832-843.
- Thomson JA et.al Journal of Food Science 66(2): 188-193.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant NPT expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
11979140
NCBI Official Name:
neomycin phosphotransferase
NCBI Official Symbol:
npt
NCBI Organism:
Plasmid pRL765
Physical State:
Liquid
Protein Accession #:
Q79C30
Protein GI Number:
81919445
Purification:
Antibody is purified by protein A affinity chromatography.
Research Area:
Signal Transduction
Swissprot #:
Q79C30
User NOte:
Optimal dilutions for each application to be determined by the researcher.