PRAK Monoclonal Antibody

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ProSci
Product Code: PSI-32-205
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-205-0.1mL0.1mL£560.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 7H10B4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
PRAK monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis using PRAK monoclonal antibody against truncated PRAK - His recombinant protein.
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Western blot analysis using PRAK monoclonal antibody against truncated PRAK - His recombinant protein.

Documents

Further Information

Additional Names:
PRAKMAPK-activated protein kinase 5, MK5, MK-5, PRAK, MAPKAP-K5
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
PRAK (p38-regulated /activated kinase), also referred to as mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK)-5, is an ubiquitously expressed serine/threonine kinase regulated by p38α and p38β MAP kinases. Activated JNK, p38? or p38? are unable to induce phosphorylation of PRAK in vitro. Phosphorylation of PRAK occurs in vivo in response to p38 activation by stress-related extracellular stimuli including UV light, oxidation and proinflammatory cytokines. Two other substrates for p38, MAPKAPK-2 and MAPKAPK-3/3pK, share approximately 45% sequence homology with PRAK including the phosphorylation motif recognized by p38, Lys-X-Thr-Pro. Activated PRAK has been shown to specifically phosphorylate HSP 27 in vitro, suggesting that the protein may play a role in stress-induced small heat shock protein phosphorylation in vivo.
Background References:
  • Paliga AJ. Natale DR. Watson AJ. Biol Cell. 2005, Aug, 97(8):629-40.
  • Wijtten PJ. Prak R. Lemme A. et al. Br Poult Sci. 2004, Aug, 45(4):504-11.
  • New L. Jiang Y. Han J. Mol Biol Cell. 2003, Jun, 14(6):2603-16. Epub 2003 Mar 20.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant PRAK-His expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
8550
NCBI Official Name:
mitogen-activated protein kinase-activated protein kinase 5
NCBI Official Symbol:
MAPKAPK5
NCBI Organism:
Homo sapiens
Protein Accession #:
Q8IW41
Protein GI Number:
52000829
Swissprot #:
Q8IW41
User NOte:
Optimal dilutions for each application to be determined by the researcher.