PSME1 Antibody

ProSci
Product Code: PSI-46-238
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-238-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-238 (0.3ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

46-238 (0.3ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
PSME1, proteasome (prosome, macropain) activator subunit 1 (PA28 alpha), IFI5111, MGC8628, PA28A, PA28alpha, REGalpha , 11S regulator complex alpha subunit, 29-kD MCP activator subunit, activator of multicatalytic protease subunit 1, interferon gamma up-regulated I-5111 protein, interferon-gamma IEF SSP 5111, interferon-gamma-inducible protein 5111, proteasome activator subunit 1, proteasome activator subunit-1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 30kDa band observed in Human Lymph Node, Spleen, Tonsil and Peripheral Blood Mononuceocyte lysates (calculated MW of 28.7kDa according to NP_006254.1). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Brychcy M, Kuckelkorn U, Hausdorf G, Egerer K, Kloetzel PM, Burmester GR, Feist E. Anti-20S proteasome autoantibodies inhibit proteasome stimulation by proteasome activator PA28. Arthritis Rheum. 2006 Jul;54(7):2175-83.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-KNFEKLKKPRGETK
NCBI Gene ID #:
5720
NCBI Official Name:
proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
NCBI Official Symbol:
PSME1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006254.1
Protein GI Number:
5453990
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise isoform 1 (NP_006254.1).
Swissprot #:
Q06323