MLL Monoclonal Antibody

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ProSci
Product Code: PSI-32-194
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-194-0.1mL0.1mL£560.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 10F8D7
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
MLL monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis using anti MLL monoclonal antibody against truncated MLL - Trx recombinant protein (1) and truncated GFP - MLL (aa3714 - 3969) grasfected COS - 7 cell lsyate (2).
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Western blot analysis using anti MLL monoclonal antibody against truncated MLL - Trx recombinant protein (1) and truncated GFP - MLL (aa3714 - 3969) grasfected COS - 7 cell lsyate (2).

Documents

Further Information

Additional Names:
ALL1, CXXC7, HRX, HTRX, MLL, MLL1, TRX1, ALL-1, Lysine N-methyltransferase 2A, HTRX1, MLL1A, WDSTS, MLL/GAS7, TET1-MLL
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Background:
Myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila).Eukaryotic RNA polymerase II mediates the synthesis of mature and functional messenger RNA. This is a multistep process, called the transcription cycle,that includes five stages: preinitiation, promoter, clearance, elongation and termination. Elongation is thought to be a critical stage for the regulation of gene expression. ELL (11-19 lysine-rich leukemia protein, also designated MEN) functions as an RNA polymerase II elongation factor that increases the rateof transcription by suppressing transient pausing by RNA polymerase II. Also, ELL is thought to regulate cellular proliferation. ELL is abundantly expressed in peripheral blood leukocytes, skeletal muscle, placenta and testis, and has lower expression in spleen, thymus, heart, brain, lung, kidney, liver and ovary.The gene encoding human ELL, which maps to chromosome 19p13.1, is one of several genes which undergo translocation with the MLL gene on chromo-some 11q23 in acute myeloid leukemia. MLL (myeloid/lymphoid leukemia,also designated ALL-1 and HRX) is a 430 kDa protein that regulates embryonal and hematopoietic development.
Background References:
  • Genet Couns. 2006;17(2):155-9.
  • Cancer Genet Cytogenet. 2006 Jul 15;168(2):162-7.
  • Leukemia. 2007 Feb;21(2):360-2. Epub 2007 Jan 4.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Purified truncated recombinant MLL-Trx (aa3751-3968) is expressed in E.Coli strain BL21 (DE3).
NCBI Gene ID #:
4297
NCBI Official Name:
lysine (K)-specific methyltransferase 2A
NCBI Official Symbol:
KMT2A
NCBI Organism:
Homo sapiens
Protein Accession #:
Q03164
Protein GI Number:
146345435
Swissprot #:
Q03164
User NOte:
Optimal dilutions for each application to be determined by the researcher.