Robo1 Antibody
Code | Size | Price |
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PSI-46-316-0.1mg | 0.1mg | £626.00 |
Quantity:
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Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
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Further Information
Additional Names:
Robo1, roundabout homolog 1 (Drosophila) , AW494633, AW742721, DUTT1 , roundabout homolog 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 200kDa band observed in fetal Mouse Brain lysates (calculated MW of 176kDa according to NP_062286.2). Recommended concentration: 0.05-0.2ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Mouse Testis. Recommended concentration: 5ug/ml.
Background References:
- Andrews W, Liapi A, Plachez C, Camurri L, Zhang J, Mori S, Murakami F, Parnavelas JG, Sundaresan V, Richards LJ. Robo1 regulates the development of major axon tracts and interneuron migration in the forebrain. Development. 2006 Jun;133(11):2243-52.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen:
The immunogen for this antibody is: C-QKARPAKKQKHQ
NCBI Gene ID #:
19876 (mouse); 58946 (rat);
NCBI Official Name:
roundabout homolog 1 (Drosophila)
NCBI Official Symbol:
Robo1
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
NP_062286.2
Protein GI Number:
122114644
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
O89026