TAK1 Antibody
| Code | Size | Price |
|---|
| PSI-46-469-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
MAP3K7 , TAK1, mitogen-activated protein kinase kinase kinase 7 , TGF1a , TGF-beta activated kinase 1, transforming growth factor-beta-activated kinase 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 75kDa band observed in lysates of cell lines HeLa, Daudi and NIH3T3, and approx. 80kDa in lysates of cell line U937 (calculated MW of 67.2 according to Human NP_663304.1 and Mouse NP_033342.1). The observed molecular weight corresponds to different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Background References:
- Omori E, Matsumoto K, Sanjo H, Sato S, Akira S, Smart RC, Ninomiya-Tsuji J. TAK1 is a master regulator of epidermal homeostasis involving skin inflammation and apoptosis. J Biol Chem. 2006 May 4;
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cow
Immunogen:
The immunogen for this antibody is: C-AELDQDEKDQQNT
NCBI Gene ID #:
6885
NCBI Official Name:
mitogen-activated protein kinase kinase kinase 7
NCBI Official Symbol:
MAP3K7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003179.1, NP_663304.1
Protein GI Number:
4507361
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise isoform A (NP_003179.1) and isoform B (NP_663304.1).
Swissprot #:
O43318