TMP21 Antibody
| Code | Size | Price |
|---|
| PSI-46-502-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
TMED10, transmembrane emp24-like trafficking protein 10 (yeast), P24(DELTA), S31I125, S31III125, TMP21, Tmp-21-I , 21 kDa transmembrane trafficking protein, transmembrane emp24 domain-containing protein 10
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Preliminary experiments gave an approx 75kDa band in Rat Pancreas lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25.0kDa according to NP_006818.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature.Immunofluorescence:Expression of the protein seen in the Golgi and cytoplasm of Glioblastoma U251 cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Background References:
- Chen F, Hasegawa H, Schmitt-Ulms G, Kawarai T, Bohm C, Katayama T, Gu Y, Sanjo N, Glista M, Rogaeva E, Wakutani Y, Pardossi-Piquard R, Ruan X, Tandon A, Checler F, Marambaud P, Hansen K, Westaway D, St George-Hyslop P, Fraser P. TMP21 is a presenilin complex component that modulates gamma-secretase but not epsilon-secretase activity. Nature. 2006 Apr 27;440(7088):1208-12.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-YLRRFFKAKKLIE
NCBI Gene ID #:
10972
NCBI Official Name:
transmembrane emp24-like trafficking protein 10 (yeast)
NCBI Official Symbol:
TMED10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006818.3
Protein GI Number:
98986464
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
P49755