Triosephosphate isomerase Antibody

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ProSci
Product Code: PSI-46-528
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-528-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-528 staining (0.003ug/ml) of Human Liver lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.
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46-528 staining (0.003ug/ml) of Human Liver lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
TPI1, triosephosphate isomerase 1, TPI, MGC88108
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 26kDa band observed in Human Liver (calculated MW of 26.7kDa according to NP_000356.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubated for 1 hour.Immunohistochemistry:In paraffin embedded Human Liver shows staining of the sinusoids. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Pancreas. Recommended concentration: 5ug/ml.
Background References:
  • Jiang PZ, Gan M, Huang H, Shen XM, Wang S, Yao KT. Proteomics-based Identification of Proteins with Altered Expression Induced by 12-O-Tetradecanoylphorbol 13-acetate in Nasopharyngeal Carcinoma CNE2 Cells. Acta Biochim Biophys Sin (Shanghai). 2005 Feb;37(2):97-106.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-LKPEFVDIINAKQ
NCBI Gene ID #:
7167
NCBI Official Name:
triosephosphate isomerase 1
NCBI Official Symbol:
TPI1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000356.1, NP_001152759.1
Protein GI Number:
4507645
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise reported isoforms 1 and 2 (NP_000356.1; NP_001152759.1).
Swissprot #:
P60174