TRPV5 Antibody

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ProSci
Product Code: PSI-46-533
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-533-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-533 (1ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-533 (1ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
TRPV5 , transient receptor potential cation channel, subfamily V, member 5 , CAT2, ECAC1, OTRPC3, calcium transporter 2, epithelial calcium channel 1, osm-9-like TRP channel 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 85kDa band observed in Human Pancreas lysates (calculated MW of 82.6kDa according to NP_062815.2). Recommended concentration: 1-3ug/ml.Immunocytochemsitry/Immunofluorescence: This antibody has been successfully used in ICC/IF for human retinal pigment epithelium as described in the following paper: Kennedy et al, Mol Vis. 2010 Apr 14;16:665-75; PMID: 20405023.
Background References:
  • van der Eerden BC, Hoenderop JG, de Vries TJ, Schoenmaker T, Buurman CJ, Uitterlinden AG, Pols HA, Bindels RJ, van Leeuwen JP. The epithelial Ca2+ channel TRPV5 is essential for proper osteoclastic bone resorption. Proc Natl Acad Sci USA. 2005 Nov 29;102(48):17507-12. Epub 2005 Nov 16.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-SHRGWEILRQNT
NCBI Gene ID #:
56302
NCBI Official Name:
transient receptor potential cation channel, subfamily V, member 5
NCBI Official Symbol:
TRPV5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_062815.2
Protein GI Number:
17505200
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9NQA5