UBE2T Antibody

ProSci
Product Code: PSI-46-546
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-546-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-546 (0.1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

46-546 (0.1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
UBE2T, HSPC150, PIG50, HSPC150 protein similar to ubiquitin-conjugating enzyme, ubiquitin conjugating enzyme, ubiquitin-conjugating enzyme E2T (putative), ubiquitin-conjugating enzyme E2T
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 22kDa band observed in lysates of cell lines K562, HEK293 and A549 (calculated MW of 22.5kDa according to NP_054895.1). Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • Machida YJ, Machida Y, Chen Y, Gurtan AM, Kupfer GM, D'Andrea AD, Dutta A. UBE2T is the E2 in the Fanconi anemia pathway and undergoes negative autoregulation. Mol Cell. 2006 Aug;23(4):589-96.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-QLVGIEKKFHPDV
NCBI Gene ID #:
29089
NCBI Official Name:
ubiquitin-conjugating enzyme E2T (putative)
NCBI Official Symbol:
UBE2T
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_054895.1
Protein GI Number:
7661808
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9NPD8