UCP1 Antibody

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ProSci
Product Code: PSI-46-548
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-548-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-548 (1ug/ml) staining of Rat Adipose (A) and (0.5ug/ml) Skeletal Muscle (B) lysate (35ug protein in RIPA buffer) Detected by chemiluminescence.
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46-548 (1ug/ml) staining of Rat Adipose (A) and (0.5ug/ml) Skeletal Muscle (B) lysate (35ug protein in RIPA buffer) Detected by chemiluminescence.

Documents

Further Information

Additional Names:
UCP1, uncoupling protein 1 (mitochondrial, proton carrier), HGNC:12517, SLC25A7, UCP, mitochondrial brown fat uncoupling protein, uncoupling protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in Rat Adipose tissue and Skeletal Muscle lysates (calculated MW of 33.2kDa according to Rat NP_036814.1). Recommended concentration: 0.5-1 ug/ml. . Primary incubation 1 hour at room temperature.
Background References:
  • Ishigaki Y, Katagiri H, Yamada T, Ogihara T, Imai J, Uno K, Hasegawa Y, Gao J, Ishihara H, Shimosegawa T, Sakoda H, Asano T, Oka Y. Dissipating excess energy stored in the liver is a potential treatment strategy for diabetes associated with obesity. Diabetes. 2005 Feb;54(2):322-32.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Dog
Immunogen:
The immunogen for this antibody is: C-EQLKRELSKSRQ
NCBI Gene ID #:
7350
NCBI Official Name:
uncoupling protein 1 (mitochondrial, proton carrier)
NCBI Official Symbol:
UCP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_068605.1
Protein GI Number:
11225256
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P25874