UCP2 Antibody

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ProSci
Product Code: PSI-46-549
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-549-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-549 (1ug/ml) staining of Rat Adipose (A) and Mouse Spleen (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-549 (3.75ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
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46-549 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI
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46-549 (1ug/ml) staining of Rat Adipose (A) and Mouse Spleen (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-549 (3.75ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
46-549 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI

Documents

Further Information

Additional Names:
UCP2, uncoupling protein 2 (mitochondrial, proton carrier), SLC25A8, UCPH , uncoupling protein 2, uncoupling protein-2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37kDa band observed in Rat Adipose and 28-30kDa in Mouse Spleen lysates (calculated MW of 33.4kDa according to Mouse NP_035801.3 and 33.4kDa according to Rat NP_062227.2). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence:?Strong expression of the protein seen in the Mitochondria of MCF7 cells. Recommended concentration: 10ug/ml.
Background References:
  • De Souza CT, Araujo EP, Stoppiglia LF, Pauli JR, Ropelle E, Rocco SA, Marin RM, Franchini KG, Carvalheira JB, Saad MJ, Boschero AC, Carneiro EM, Velloso LA. Inhibition of UCP2 expression reverses diet-induced diabetes mellitus by effects on both insulin secretion and action. FASEB J. 2007 Jan 5;
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-DSVKQFYTKGSEH
NCBI Gene ID #:
7351
NCBI Official Name:
uncoupling protein 2 (mitochondrial, proton carrier)
NCBI Official Symbol:
UCP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003346.2
Protein GI Number:
13259541
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P55851