VPS41 Antibody
| Code | Size | Price |
|---|
| PSI-46-586-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
VPS41, vacuolar protein sorting 41 (yeast) , HGNC:12713, HVSP41, vacuolar assembly protein 41, vacuolar protein sorting 41 (yeast homolog), vacuolar protein sorting 41 homolog (S. cerevisiae), HVPS41, hVps41p, vacuolar protein sorting 41
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave bands at approx 48kDa and 28kDa in human duodenum, kidney and placenta lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 98.5kDa according to NP_055211.1 and 100kDa according to NP_542198.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).Immunohistochemistry:In paraffin embedded Human Kidney shows staining of collective tubules. Recommended concentration: 4-6ug/ml. Paraffin embedded Human Placenta and Prostate. Recommended concentration: 3.75ug/ml.
Background References:
- McVey Ward D, Radisky D, Scullion MA, Tuttle MS, Vaughn M, Kaplan J. hVPS41 is expressed in multiple isoforms and can associate with vesicles through a RING-H2 finger motif. Exp Cell Res. 2001 Jul 1;267(1):126-34.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-DHLKKDSQNKTLLK
NCBI Gene ID #:
27072
NCBI Official Name:
vacuolar protein sorting 41 homolog (S. cerevisiae)
NCBI Official Symbol:
VPS41
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055211.2, NP_542198.2
Protein GI Number:
7657677
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_055211.2 and NP_542198.2)
Swissprot #:
P49754