VPS45 Antibody

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ProSci
Product Code: PSI-46-587
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-587-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-587 (0.3ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-587 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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46-587 (0.3ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-587 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
VPS45, VPS45A, VPS45B, VSP45, VSP45A, H1, H1VPS45, leucocyte vacuolar protein sorting 45, vacuolar protein sorting 45A, vacuolar protein sorting 45A (yeast homolog), vacuolar protein sorting 45 homolog (S. cerevisiae), vacuolar protein sorting 45A (yeast), vacuolar protein sorting 45B (yeast), vacuolar protein sorting protein similar to S. cerevisiae Vsp45p, RP11-458I7.2, VPS54A
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in Human Testis and Peripheral Blood Mononucleocyte lysates (calculated MW of 65.1kDa according to NP_009190.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex), Placenta and Small Intestine. Recommended concentration: 5ug/ml.
Background References:
  • Rajasekariah P, Eyre HJ, Stanley KK, Walls RS, Sutherland GR. Molecular cloning and characterization of a cDNA encoding the human leucocyte vacuolar protein sorting (h1Vps45). Int J Biochem Cell Biol. 1999 Jun;31(6):683-94.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-ESSQVTSRSASRR
NCBI Gene ID #:
11311
NCBI Official Name:
vacuolar protein sorting 45 homolog (S. cerevisiae)
NCBI Official Symbol:
VPS45
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_009190.2
Protein GI Number:
18105063
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9NRW7