Annexin A1 Antibody

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ProSci
Product Code: PSI-46-785
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-785-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-785 (1ug/ml) staining of K562 (A) and NIH3T3 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
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4 / 5
46-785 (4ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
5 / 5
46-785 (4ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
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46-785 (1ug/ml) staining of K562 (A) and NIH3T3 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
46-785 (4ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
46-785 (4ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>

Documents

Further Information

Additional Names:
annexin I, ANXA1, annexin A1, ANX1, LPC1, annexin I (lipocortin I), lipocortin I
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 37kDa band observed in lysates of cell lines K562 and NIH3T3 (calculated MW of 38.7kDa according to Human NP_000691.1 and Mouse NP_034860.2). Approx 37kDa band observed in Rat and Pig Spleen lysates (calculated MW of 38.7kDa according to Pig NP_001157470.1). Recommended concentration: 1-3ug/ml.
Background References:
  • Fan X, Krahling S, Smith D, Williamson P, Schlegel RA. Macrophage surface expression of annexins I and II in the phagocytosis of apoptotic lymphocytes. Mol Biol Cell. 2004 Jun;15(6):2863-72. Epub 2004 Apr 2.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: CQAILDETKGDYEK
NCBI Gene ID #:
301
NCBI Official Name:
annexin A1
NCBI Official Symbol:
ANXA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000691.1
Protein GI Number:
4502101
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
.
Swissprot #:
P04083