ZNF217 Antibody

ProSci
Product Code: PSI-46-616
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-616-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-616 (3.8ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

46-616 (3.8ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
ZNF217, zinc finger protein 217, ZABC1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 75kDa band in Human Prostate, Spleen, Testis and Thymus lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 115kDa according to NP_006517.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Prostate shows nuclear staning in select secretory cells. Recommended concentration, 3-6ug/ml.
Background References:
  • Huang G, Krig S, Kowbel D, Xu H, Hyun B, Volik S, Feuerstein B, Mills GB, Stokoe D, Yaswen P, Collins C. ZNF217 suppresses cell death associated with chemotherapy and telomere dysfunction. Hum Mol Genet. 2005 Nov 1;14(21):3219-25. Epub 2005 Oct 3.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-KNAADDSADKVNKN
NCBI Gene ID #:
7764
NCBI Official Name:
zinc finger protein 217
NCBI Official Symbol:
ZNF217
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006517.1
Protein GI Number:
5730124
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
O75362