E2F3 Antibody
| Code | Size | Price |
|---|
| PSI-28-786-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Canine (dog)
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store E2F3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
E2F3, E2F-3
Application Note:
E2F3 antibody can be used for detection of E2F3 by ELISA at 1:62500. E2F3 antibody can be used for detection of E2F3 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
E2F3 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. E2F3 binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.
Background References:
- Koehrer, K., et al., (2004) The German cDNA Consortium.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human E2F3.
NCBI Gene ID #:
1871
NCBI Official Name:
E2F transcription factor 3
NCBI Official Symbol:
E2F3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
37 kDa, 49 kDa
Protein Accession #:
CAH18140
Protein GI Number:
51476300
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Transcription ,Cell Cycle ,Cancer
Swissprot #:
Q68DT0
User NOte:
Optimal dilutions for each application to be determined by the researcher.