GFI1B Antibody

ProSci
Product Code: PSI-27-287
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-27-287-100ul100ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store GFI1B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in IHC on Human kidney at 4.0-8.0 ug/ml.
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Antibody used in WB on Human Jurkat 0.2-1 ug/ml.

Antibody used in IHC on Human kidney at 4.0-8.0 ug/ml.
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.

Further Information

Additional Names:
GFI1B, BDPLT17
Application Note:
GFI1B antibody can be used for detection of GFI1B by ELISA at 1:312500. GFI1B antibody can be used for detection of GFI1B by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
GFI1B acts in the late stage of erythroid differentiation as a transcriptional repressor. GATA-1 and NF-Y both contribute to erythroid-specific transcriptional activation of the Gfi-1B promoter. This zinc finger protein mediates erythroid expansion and has a role in normal erythropoiesis.
Background References:
  • Huang, D.Y., et al., (2004) Nucleic Acids Res. 32 (13), 3935-3946.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GFI1B.
NCBI Gene ID #:
8328
NCBI Official Name:
growth factor independent 1B transcription repressor
NCBI Official Symbol:
GFI1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
NP_004179
Protein GI Number:
205277343
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Transcription ,Cell Cycle ,Cancer
Swissprot #:
Q6T888
User NOte:
Optimal dilutions for each application to be determined by the researcher.