USP11 Antibody

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ProSci
Product Code: PSI-46-555
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-555-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-555 staining (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
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46-555 staining (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
USP11, UHX1, ubiquitin specific protease 11, deubiquitinating enzyme 11, ubiquitin thiolesterase 11, ubiquitin carboxyl-terminal hydrolase, X-linked, ubiquitin specific peptidase 11, RP4-659F15.2, ubiquitin-specific processing protease 11
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 120kDa band observed in lysates of cell line Jurkat (calculated MW of 110kDa according to NP_004642.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ideguchi et al, Biochem J. 2002 Oct 1;367(Pt 1):87-95.; PMID: 12084015). Recommended concentration: 1-3ug/ml.
Background References:
  • Ideguchi H, Ueda A, Tanaka M, Yang J, Tsuji T, Ohno S, Hagiwara E, Aoki A, Ishigatsubo Y. Structural and functional characterization of the USP11 deubiquitinating enzyme, which interacts with the RanGTP-associated protein RanBPM. Biochem J. 2002 Oct 1;367(Pt 1):87-95.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: CSSPPSSEFMDVN
NCBI Gene ID #:
8237
NCBI Official Name:
ubiquitin specific peptidase 11
NCBI Official Symbol:
USP11
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004642.2
Protein GI Number:
24234683
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P51784